Journal: Osteoarthritis and cartilage

Article Title: Osteoarthritic synovial fibroblasts drive inflammatory responses and impair chondrocyte function in response to a fibronectin matrikine

doi: 10.1016/j.joca.2026.04.011

Figure Lengend Snippet: FN7–10-induced changes in synovial fibroblast pro-inflammatory protein secretion. (A) Schematic timeline showing synovial fibroblast serum starvation, stimulation with 1 μM FN7–10, and conditioned media collection for cytokine array analysis (created with BioRender). (B) Representative cytokine arrrays incubated with conditioned media (CM) from synovial fibroblast treated with PBS (left) or FN7–10 (right). Red arrows highlight cytokines detected in the control condition (PBS CM) and increased by at least 1.5-log2fold following FN7–10 stimulation. Positive controls for the array are labeled “POS”. (C) Normalized densitometric quantification of selected cytokines from PBS CM, showing individual donor values with mean ± 95% confidence intervals (n = 4 independent synovial fibroblast donors). (D) Densitometry-based log 2 fold change of selected cytokines detected in the array comparing FN7–10 CM to PBS CM, displayed as individual donor values with mean ± 95% confidence intervals.

Article Snippet: The C5 human cytokine protein array (RayBiotech, AAH-CYT-5–2) was used to evaluate the secreted proteins found in the CM.

Techniques: Incubation, Control, Labeling

Journal: Osteoarthritis and cartilage

Article Title: Osteoarthritic synovial fibroblasts drive inflammatory responses and impair chondrocyte function in response to a fibronectin matrikine

doi: 10.1016/j.joca.2026.04.011

Figure Lengend Snippet: FN710–induced changes in synovial fibroblast cytokine and chemokine gene expression. (A) Boxplot showing the log 2 fold change of significantly regulated genes (padj < 0.05) within the KEGG cytokine–cytokine receptor interaction pathway (hsa04060), grouped by major cytokine families. Chemokine datapoints with |log 2 FC= > 4 are bolded and labeled in black, and the horizontal dashed line indicates no change (log 2 FC = 0). (B) Targeted heatmap illustrating expression of chemokine family genes identified in panel A, comparing synovial fibroblasts treated with PBS or FN710 for 18 h (n = 5 independent OA synovial fibroblast donors). Values represent relative RNA expression (FN7–10/PBS).

Article Snippet: The C5 human cytokine protein array (RayBiotech, AAH-CYT-5–2) was used to evaluate the secreted proteins found in the CM.

Techniques: Gene Expression, Labeling, Expressing, RNA Expression

Journal: Oncotarget

Article Title: Dual inhibition of HDAC and EGFR signaling with CUDC-101 induces potent suppression of tumor growth and metastasis in anaplastic thyroid cancer.

doi: 10.18632/oncotarget.3268

Figure Lengend Snippet: Figure 3: CUDC-101 inhibits ATC cell proliferation, and induces cell cycle arrest and apoptosis. (A) Basal expression of HDAC1, HDAC2 and EGFR in ATC cell lines. (B) Cell proliferation assay. Error bars are mean ± SD. (C) Cell cycle analysis after 24 hours of treatment. (D) The Caspase-Glo 3/7 assay after 48 hours of treatment with CUDC-101. *p < 0.05, **p < 0.01, ***p < 0.001. NS, no significant difference.

Article Snippet: Phospho-kinase and apoptosis protein arrays Human phospho-kinase (Catalog # ARY003B) array (detecting site-specific phosphorylation of 43 kinases and 2 related total proteins, and apoptosis array (Catalog # ARY009 detecting the expression level of 35 apoptosisrelated proteins) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Expressing, Proliferation Assay, Cell Cycle Assay, Caspase-Glo Assay

Journal: Oncotarget

Article Title: Dual inhibition of HDAC and EGFR signaling with CUDC-101 induces potent suppression of tumor growth and metastasis in anaplastic thyroid cancer.

doi: 10.18632/oncotarget.3268

Figure Lengend Snippet: Figure 5: Protein targets of CUDC-101 in ATC cells. (A) CUDC-101 inhibits HDACs and MAPK in ATC cells. (B) Targets of CUDC-101 identified by phospho-kinase array. Phospho-kinase arrays were performed using cell lysates treated with and without CUDC-101. Shown are those proteins that were altered with CUDC-101 treatment in both ATC cell lines with > 1.5-fold difference. (C) Differentially expressed apoptotic proteins with and without CUDC-101 treatment. Apoptosis arrays were performed. Shown are those proteins that were altered with CUDC-101 treatment in both ATC cell lines with > 1.5-fold difference. For A–C, ATC cells were treated with the vehicle or CUDC-101 at 1.1 μM for 24 hours. (D) CUDC-101 reduces the expression of survivin, XIAP, and β-catenin. Cells were treated with the vehicle or CUDC-101 for 24 hours.

Article Snippet: Phospho-kinase and apoptosis protein arrays Human phospho-kinase (Catalog # ARY003B) array (detecting site-specific phosphorylation of 43 kinases and 2 related total proteins, and apoptosis array (Catalog # ARY009 detecting the expression level of 35 apoptosisrelated proteins) were purchased from R&D Systems (Minneapolis, MN).

Techniques: Expressing